L6397

Sigma

 

Concanavalin A from Canavalia ensiformis (Jack bean)

peroxidase conjugate, lyophilized powder

Synonym:Con A
CAS Number:11028-71-0
MDL number:MFCD00071069

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Description

Analysis NoteWhere reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
ApplicationDetects glycoproteins containing α-D-mannose, α-D-glucose when used with appropriate peroxidase substrate
Biochem/physiol ActionsCon A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
PackagingPackage size based on protein content
Preparation NotePrepared from peroxidase type VI (P8375), using the method of Wilson and Nakane,1 which promotes conjugation but prevents the interaction between Con A and peroxidase sugar residues.
Unit DefinitionOne unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.
Physical formLyophilized powder containing tris-citrate buffer salts and trace calcium and manganese

Properties

formlyophilized powder
total impurities carbohydrate, essentially free
conjugateperoxidase conjugate
storage temp.−20°C

Safety

Personal Protective Equipmentdust mask type N95 (US), Eyeshields, Faceshields, Gloves
Hazard CodesXn
Risk Statements20/21/22-40-42/43
Safety Statements36
WGK Germany3

References

Cited Reference1. Wilson, M.B. and Nakane, P.K., Knapp, P., ed. Immunofluorescence and Related Staining Techniques, (1978), 215
referenceReeke, G.N., et al. Ann. N.Y. Acad. Sci. 234, 369, (1974)
 Kalb, A.J. and Lustig, A. Biochim. Biophys. Acta 168, 366, (1968)
 Gunther, G.R., et al. Proc. Natl. Acad. Sci. U. S. A. 70, 1012, (1973)
 , Bittiges, H. and Schnebli, H.P, ed. Concanavalin A as a Tool London , (1976)
MerckMerck 13,2521